The study aims to explore the accumulation of nitrogen in rice plants by different planting methods，and in order to provide theoretical and technical support for the scientific management of water nitrogen in double-cropping rice.A two-year field experiment was conducted from 2020 to 2021，consisting of four treatments：direct seeding（DS），machine transplanting（MT），seedling-throwing（ST），and hand transplanting（HT），and the change characteristics of nitrogen distribution and accumulation in rice seedling stage （from sowing to transplanting），field stage（from transplanting to harvesting）and whole growth period （from sowing to harvesting）were studied under multiple planting methods.The results showed that the grain-straw ratio and nitrogen absorption of above-ground plant parts of HT was the largest after the early and late rice harvest in the rice seedling stage，the field stage and the whole growth period ，but DS was the least.In early rice，HT was higher than ST and MT and DS by 0.21% -7.82% 4.75% -24.05% 48.34%-63.27% respectively；but the late rice was higher by 37.76%-78.05%、12.77%-12.31%、21.23%-39.40%.moreover，the grain harvest index（GHI）and nitrogen harvest index（NHI）of early and late rice were the largest by HT and the smallest by DS，and there were significant differences between HT，MT，ST and DS.Above all，DS will reduce the absorption of nitrogen in double cropping rice plants，and HT is more conducive to improve the utilization efficiency of nitrogen，and the difference of nitrogen accumulation and utilization among the four planting methods provides a theoretical reference for the sustainable development of rice production.