In this paper,the output coefficient method is used to analyze the amount of totalnitrogen and totalphosphorus into the river of agricultural non-point source pollution in Xiannv lake basin.The results show that the main agricultural pollution sources in the basin are livestock and poultry pollution and farmland fertilizer loss,and the proportion of total nitrogen and total phosphorus into the river is more than 80%.Yuanzhou district is the main pollution source of total nitrogen and total phosphorus in the basin,and the proportion of total nitrogen and total phosphorus into the river is 59%.