Recently，a large number of pollutants resulting from frequent oil spill incidents pose great threats to water environment.And there were many studies focused on the oil spill pollutants remove.Currently，physical and chemical methods are still the top choices to deal with oil spill pollution.However，they cannot fundamentally remove the oil pollutants from water body，and may even cause secondary pollution in the long run.With advantages as low toxicity and environment-friendly，biological method is considered to be the most potential value on oil spill treatment currently.Bio-surfactant occupies the core status in the process of dealing with oil spill pollutants in biological method.Bio-surfactant and its application in the oil spill incidents is discussed in this article，which could provide some reference for oil spill emergency treatment or final disposal.