Water diversion project is an important method to realize the optimal allocation of water resources, keep ecological balance and maintenance of ecological health. Karamay diversion project as a diversion project built in the northwest cold area of China, the water diversion project has profoundly changed the ecological landscape of Karamay, and the society and the economy have been developing rapidly. Agricultural Development Zone as an important measure of the transformation and upgrading of Karamay, long-term irrigation caused the continuous uplift of the groundwater level, has far exceeded the Karamay groundwater safety critical water depth of the redline. This paper introduces the basic situation in Karamay on the basis of research on groundwater in agricultural development area of variation of deep buried dynamic analysis, analysis on the relationship between irrigation water and groundwater level, and puts forward the countermeasures and suggestions of ecological regulation for decision.