The multistage centrifugal pump is widely applied because of its high effectiveness and lift. However, it also has several disadvantages, namely complex structure, and high-speed rotation of rotor during operation which can easily induce vibration, conduct damage and influence the safety operation. Employing ANSYS Workbench, together with simulation software, to conduct modal analysis toward the rotor of multistage centrifugal pump, so as to gain the intrinsic frequency and vibration model of the first ten steps. Researching the vibration characteristics of rotor system, examining the influence of impeller progression on the modal, as well as analyzing and anticipating the possibility of that impeller progression change induces vibration. The results show that: the D-type five-progression centrifugal pump has good vibration characteristics but no resonance under give operation condition. The increase of impeller progression will increase the possibility of inducting resonance that the operation shall keep away from the rotate speed range which can easily induce resonance. The analysis results lay theoretical foundation for the vibration fault diagnosis and anticipation of rotor system, optimization design of dynamic characteristics as well as dynamics, and have significant meanings toward decreasing vibration, avoiding resonance as well as keeping safety and stable operation.