Public satisfaction of ecosystem service in Poyang Lake was investigated and analyzed with method of analytic hierarchy process (AHP) through questionnaire survey. Results showed that public satisfaction of the ecosystem service were moderate in Poyang Lake, with proportion of 51.8%. Publics were not satisfied in aspects of sand mining quantity, flood control capacity, water quality, number of cowfish and crane, wetland area, and habitat function. Therefore, it is of great significance to improve ecosystem service in the Poyang Lake, i.e., controlling the amount of sand mining and crack down on illegal sand mining, improving the flood control ability and water quality of Poyang Lake, strengthening protection of endangered species and habitat in this lake, and returning arable land to this lake.